ISCE High‑Performance Capillary Electrophoresis System


Explore the molecular world, pursue analytical perfection
Product Introduction
The ISCE‑1000 Capillary Electrophoresis System is a new‑generation analytical instrument developed based on high‑performance capillary electrophoresis technology. Adopting intelligent design and advanced control systems, it achieves industry‑leading levels in separation efficiency, detection sensitivity and operation convenience. Featuring a modular design, the instrument supports optional multiple detectors and injection modes to meet analytical requirements in diverse fields.
High‑performance capillary electrophoresis is an analytical technique featuring high efficiency, fast speed and high sensitivity. Driven by an electric field, charged particles migrate inside the capillary. Rapid sample separation and detection are realized based on differences in substance charge, molecular size, isoelectric point (pI), hydrophobicity and other properties. The ISCE‑1000 Capillary Electrophoresis System is a product launched by Insysis after years of technical accumulation. Its performance benchmarks international leading brands, with advancements in intelligence, regulatory compliance and application support.
It is widely applied in biochemistry, pharmaceuticals, environmental science, food safety and other fields. It is especially suitable for separation and analysis of biomolecules such as proteins, peptides, nucleic acids and saccharides, as well as chiral compounds and small‑molecule compounds.
Product Features

1. Outstanding separation performance: Continuously adjustable high‑voltage output of 0±30 kV, paired with a precise liquid‑cooling temperature‑control system (10‑60 °C), ensures high resolution and excellent repeatability.
2. Flexible separation modes: Supports multiple separation modes — capillary zone electrophoresis, capillary gel electrophoresis, capillary isoelectric focusing, micellar electrokinetic capillary chromatography, capillary electrochromatography, etc.
3. Intelligent operating system: Fully‑automatic sample injection, separation and data processing. The 48‑position sample tray enables continuous testing of large‑batch samples.
4. Strict regulatory compliance: The software platform fully meets 21 CFR Part 11 requirements, equipped with complete audit trail and electronic signature functions.
5. Economical operating costs: Nanoliter‑scale injection volume and milliliter‑scale solvent consumption greatly reduce experimental costs.

Main Specifications

Parameter Category	Detailed Specification
Overall Dimension	73×65×72 cm
Weight	85 kg
Power Requirement	100‑240 VAC, 50/60 Hz, 500 W
Output Voltage	0‑±30 kV continuously adjustable
Output Current	0‑300 μA
Injection Mode	Voltage: 0‑±30 kV; Pressure: 0‑100 psi continuously adjustable output
Temperature‑Control System	Liquid cooling, 10‑60 °C
Sample Capacity	1×48‑vial sample tray
Detector	UV detector, wavelength range 190‑600 nm

Typical Application Scenarios
1. CZE Detection of Aprotinin
‑ Separation Mode: Capillary Zone Electrophoresis (CZE) ‑ Capillary Specification: Fused‑silica capillary ‑ Total Length / Effective Length: 57 cm / 50 cm ‑ Inner Diameter: 75 μm ‑ Injection Condition: 0.21 psi, 3 s ‑ Separation Voltage: 12 kV ‑ Separation Buffer: 120 mM potassium dihydrogen phosphate buffer, pH 2.5 ‑ Capillary Temperature: 25 °C ‑ Sample Chamber Temperature: RT (Room Temperature) ‑ Detection Wavelength: 214 nm ‑ Sample Information: Aprotinin

 Aprotinin Detection per Chinese Pharmacopoeia — System Suitability Requirements In the electropherogram of reference solution, the migration‑time ratio of des‑Ala‑des‑Gly‑aprotinin peak relative to aprotinin peak is 0.98; the migration‑time ratio of des‑Ala‑aprotinin peak relative to aprotinin peak is 0.99. The resolution between des‑Ala‑des‑Gly‑aprotinin peak and des‑Ala‑aprotinin peak shall be greater than 0.8; the resolution between des‑Ala‑aprotinin peak and aprotinin peak shall be greater than 0.5. The tailing factor of the aprotinin peak shall not exceed 3.

Compound Name	Retention Time [min]	Peak Area (mAu*min)	Peak Area [%]	Tailing Factor	Resolution
Des-Ala-des-Gly-aprotinin	11.206	0.2	7.3	1.25	——
Des-Ala-aprotinin	11.331	0.193	7.04	1.3	1.34
Aprotinin	11.444	2.346	85.66	2.77	0.89

 
2. Non‑Reduced Monoclonal Antibody Detection by CES‑SDS
‑ Separation Mode: Capillary Gel Electrophoresis (CGE)
‑ Capillary Specification: Fused‑silica capillary
 ‑ Total Length / Effective Length: 33 cm / 26 cm
‑ Inner Diameter: 50 μm ‑ Injection Condition: 10 kV, 40 s
 ‑ Separation Voltage: 15 kV
‑ Separation Buffer: Self‑prepared mAb separation gel
‑ Capillary Temperature: 25 °C
‑ Sample Chamber Temperature: 15 °C
‑ Detection Wavelength: 220 nm
‑ Sample Information: Non‑reduced monoclonal antibody

3. Reduced Monoclonal Antibody Detection by CES‑SDS
‑ Separation Mode: Capillary Gel Electrophoresis (CGE)
‑ Capillary Specification: Fused‑silica capillary
‑ Total Length / Effective Length: 33 cm / 26 cm
‑ Inner Diameter: 50 μm
‑ Injection Condition: 10 kV, 30 s
 ‑ Separation Voltage: 15 kV
‑ Separation Buffer: Self‑prepared mAb separation gel
‑ Capillary Temperature: 25 °C
‑ Sample Chamber Temperature: 15 °C
‑ Detection Wavelength: 220 nm
‑ Sample Information: Reduced monoclonal antibody

4. CZE Detection of Lysozyme
‑ Separation Mode: Capillary Zone Electrophoresis (CZE)
‑ Capillary Specification: Fused‑silica capillary
 ‑ Total Length / Effective Length: 47 cm / 40 cm
‑ Inner Diameter: 75 μm ‑ Injection Condition: 5 kV, 5 s
‑ Separation Voltage: 10 kV
‑ Separation Buffer: 50 mM sodium dihydrogen phosphate buffer, pH 2.24
‑ Capillary Temperature: 25 °C
‑ Sample Chamber Temperature: RT (Room Temperature)
‑ Detection Wavelength: 214 nm ‑ Sample Information: Lysozyme

5. CZE Detection of Mixed Small‑Molecule Standards
‑ Separation Mode: Capillary Zone Electrophoresis (CZE)
 ‑ Capillary Specification: Fused‑silica capillary
‑ Total Length / Effective Length: 47 cm / 40 cm
‑ Inner Diameter: 75 μm
‑ Injection Condition: 0.5 psi, 5 s
 ‑ Separation Voltage: 15 kV
‑ Separation Buffer: 30 mM Na₂B₄O₇
‑ Capillary Temperature: 25 °C
 ‑ Sample Chamber Temperature: RT (Room Temperature)
‑ Detection Wavelength: 254 nm
‑ Sample Information: 0.1 mg/mL p‑nitrophenol + 0.1 mg/mL m‑nitrophenol + 0.1 mg/mL p‑nitrobenzoic acid

Notes:

1. RT = Room Temperature
2. mAb = monoclonal antibody
3. CZE = Capillary Zone Electrophoresis; CGE = Capillary Gel Electrophoresis; CES‑SDS = Capillary Electrophoresis‑Sodium Dodecyl Sulfate

Welcome to inquire by phone: E-Mail: pangjun@costones.com
 

Suzhou COSTONES Instruments Co., Ltd. (COSTONES) is an instrument company focusing
on separation and purification. It is an equipment supplier and service provider
for biopharmaceutical, cell therapy and gene testing. It is committed to providing
users with industry-leading technical support and services.
The company has a perfect service system and executive power. It interprets the
user experience from the four aspects of technical communication, scheme design,
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For a long time, we have maintained good cooperative relations with domestic and
foreign suppliers. The main products include protein purification system,
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At present, Suzhou quarrying has provided high-quality services for
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It has carried out in-depth cooperation with customers and become a reliable partner
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