A reliable immunological assay demands reliable antibodies and antibody services. ShineGene provides a one-stop solution to all our customers'' antibody needs. Our custom antibody services include recombinant protein expression, peptide design and synthesis, polyclonal antibody production, express polyclonal antibody production, affinity purification, conjugation, fragmentation, and immunoassay development.

Phase 1. Peptide synthesis

Assistance on designing peptide with respect to protein homology, antigenicity, hydrophilicity, and synthetic suitability.Free to design antigen.

Custom FMOC Peptide synthesis up to 30 amino acids (10 mg). 4 mg of free peptide will be delivered to the customer together with the antibody.

A cysteine will be automatically added to the C-terminus or N-terminus of the peptide, and conjugated to Map,KLH or BSA.Note:MAP technology is an excellent substitute for conventional carriers when your sequence is very hydrophobic, contains internal cysteines, has less than 10 amino acids in the sequence or when a stronger antigenic presentation in the animal is needed. MAP technology can be used in any antibody protocol.

Peptide purity is >80%.
Time: 2~3 weeks
Price: around EUR15.00 for each amino acid residue
(reference to our website: http://www.synthesisgene.com/peptidesynthesis.html)


Phase 2. Peptide conjugation to MAP,KLH or BSA
Conjugation of 5 mg peptide to MAP,KLH or BSA using terminal Cysteine.
Time: 3 days
Price: around EUR120.00


Phase 3. Immunization and anti-sera production in rabbit
Conjugated peptides are used to immunize rabbits.
Ten-week protocol to produce antibodies in 2 SPF rabbits of each peptide.
ELISA tests until the titer reaches 1:4,000.
1ml of pre-immune serum and 50~80ml of final immune bleeds.
Final product ELISA titer>1:8000
Time: 10 weeks
Price: EUR330.00

Phase 4. Antibody purification (optional)
Purification of the antibody by protein A or antigen-affinity resin.
Antibody will be dialyzed against PBS after purification if required.
Time: 3 days
Price: EUR120.00 for protein A purification
EUR240.00 for antigen-affinity purification
Total Time: around 14~16 weeks

ShineGene Bio-Technologies, Inc., located in Shanghai, P.R. China, is a bio-company specialized in providing bio- technical services including Gene Synthesis,Peptide Synthesis,Real Time PCR, Fluorogenic Probe Design,Fluorogenic Probe Label,HRP Enzyme Stabilizer,X-gal,IPTG,ProClin300etc. and supplying reagents such as Hotstart Taq polymerase, dNTPs, qPCR mastermix,one step qPCR kits,First Strand cDNA Synthesis Kits,etc. ShineGene also undertake some OEM or OED orders as well as production task.ShineGene works at the cutting edge of development in PCR, quantitative real-time PCR, transposon and RNA technologies. ShineGenes mission is to provide new tools of excellent quality and extreme purity to the scientific community. Therefore research and development is a continuous process in our company.

http://www.synthesisgene.com
http://www.shinegene.org.cn/englsih