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Protein Primary Structure Analysis
Protein Primary Structure Analysis
Origin of place China
Model Protein and Peptide Medicinal Analysis
Supplier Shanghai Applied Protein Technology Co. Ltd
Price
Hits 1930
Updated 5/9/2013
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Method:mass spectrometry

Principle:
Confirm the structure of protein by matching theoratical sequence and peptides coverage

Equipment:
Finnigan LCQ Deca Xp / Finnigan LTQ

Samples Requirement:
1. Salt concentration: Volatile salt<20mM, nonvolatile salt<5mM
2. Protein amount: >50pmol; purity>90%

Example:


Fig1: total ion chromatogram Fig2: peptides coverage

Result:
Sequence of the sample is Ac-SDXXVDTXXEIXXKDLKEXXVVXXAEN (X represents some kind of amino acid, in secret)
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