N-terminal sequencing
Principle：
The N-terminal sequencing is based on Edman degradation. There are three steps in each cycle: Coupling of PITC with the amino-terminal residue, Cleavage of the amino-terminal residue via cyclization in acidic media, and conversion of the ATZ derivative formed to the more stable PTC derivative, which may be identified by chromatography
Equipments： ABI491A protein sequencer
Samples Requirements：
1. Protein samples could be in the forms of dry powder, aqueous or PVDF membrane (if on membrane, protein should be less than 40KDa and stained with Ponceaus)
2. Protein amount and purity: amount>10pmol; purity>90%; concentration>1mg/ml
3. Salt concentration: Volatile salt<20mM, nonvolatile salt<5mM,Tris buffer is refusedused
4. Please inform us of the scope of the Molecular Weight
Example
Result：
N-terminal Sequencing: NH2NSXXECPXSH (X represent some kind of amino acid, in secret)
bio-equip.cn
Shanghai Applied Protein Technology Co. Ltd is a biotech company to provide customer service and product development in the proteomics filed.
The company is founded and owned by Research Center of Shanghai Institutes of Biological Sciences , Chinese Academy of Sciences.Our mission is to provide a total solution to our clients for their proteomics applications.
Through the cooperation with the proteomics center of SIBS we have developed innovate technologies and proteomics research platforms to provide the best service for our clients in the basic research, drug discovery & development, molecular diagnostics and other life sciences areas. We are the contributor to the life science.
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