Chondroitin sulfate degrades chondroitin sulfate A, chondroitin sulfate B (dermatan sulfate), and chondroitin sulfate C. This enzyme acts on and cleaves the β1-4 glycosidic bond between the disaccharide repeating units through a β-elimination mechanism. The degradation products are mainly unsaturated disaccharides, which can be detected by UV photometer (232 nm).

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