Recombinant Trypsin Pharmacopoeia grade(Freeze-dried powder)

Recombinant Trypsin 

Specifications：10mg；100mg；1g；packing specifications can be customized according to customer needs

 

I.Product introduction

Recombinant Trypsin: CAS: 9002-07-7; EC: 3.4.21.4

Recombinant Trypsin is a serine protease, which can specifically cleave the C-terminal peptide end of lysine and arginine.

Trypsin is widely used in various biotechnology processes. For example: proteolysis to produce polypeptides; cell separation of various tissues; proteolysis and sequencing; recombinant insulin production and so on.

 

II. Product characteristics

Source: Pichia pastoris

Character: white or similar white freeze-dried powder

Purity :(RP-HPLC): β-trypsin≥70%，α-trypsin ≤20%

Specific activity: ≥ 3800 USP units / mg Pro

Host protein residue: ≤0.01%

Host cell DNA residue: ≤ 10 ng/mg

Specificity: cleavage of the carboxy terminal peptide bond between arginine and lysine.

 

III. Product use

Production of recombinant insulin and analogs; cell culture and fermentation; enzymatic hydrolysis of proteins; cell separation of various tissues.

 

Iv. Recommend Usage

 It is recommended to use 1mM HCl solution to dissolve, The ration to aimed protein is 1:50 to 1:1000, optimal ph 7.0-9.0, optimal temperature 25-35℃.

Note: The activity is inhibited by serine protease inhibitors such as PMSF, metal ion chelating agents such as EDTA, etc.; high concentration of inorganic salts will have certain effects on enzyme activity.

 

V. Storage stability

The lyophilized powder can be stored for more than 2 years at temperatures below –15 °C.

 

Vi. Product advantages

Animal origin free: Recombinant production, no exogenous virus contamination. No animal-derived material is used during production.

Stable quality: stable and continuous batch production can be guaranteed; There is no difference between product batches and the quality is stable.

Lyophilized powder: easy to store and transport.

Hangzhou Putai Biotechnology Co., Ltd., located in Xuxian Industrial Park, Xiaoshan, Hangzhou, is a new high-tech enterprise established in 2018. The company takes "using genetic engineering technology to accelerate the development of innovative pharmaceutical products" as its business mission, and "customer-centric, integrity as the cornerstone, quality as the fundamental" as its core values.

In 2011, the founding shareholder of Putai bio established health path Pharma Co., Ltd., a manufacturing enterprise in Cambodia, which is now one of the most important pharmaceutical companies in Cambodia. On the one hand, Putai biology is a pilot research and development base of health path Pharma in China, on the other hand, it will provide various gene recombination products and technical services for domestic and foreign counterparts.

At present, the products of Putai bio have been developed and put into production include: recombinant trypsin, recombinant enterokinase, recombinant lysine endonuclease, recombinant carboxypeptidase B and other enzyme products; recombinant GM-CSF, recombinant G-CSF, recombinant interferon and other cytokine products.

Putai biology has a complete R & D, production and quality management system. The company has more than tons of fermentation tanks and supporting separation, purification, verification and other equipment and facilities, which can meet the needs of industrial customers.

At present, Putai biology has carried out scientific research cooperation and personnel training with Zhejiang University, Zhejiang University of science and technology, Zhejiang University of science and technology and other universities, and is committed to building an excellent scientific research and development team, contributing to the development of biological pharmaceutical industry at home and abroad.