MHC tetramer is a complex of 4 MHC molecules associated with loaded peptide, which is bound to a fluorescently labeled streptavidin molecule. Monomeric MHC molecule is loaded with its cognate peptide, and biotin is linked to the complex at a site far removed from the T-cell recognition site. The monomeric MHC-peptide complexes bind to streptavidin, which further tightly binds biotin at 4 separate sites, thus making a tetrameric complex, the MHC tetramer. https://www.creative-peptides.com/services/custom-mhc-peptides-tetramer-service.html

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