Introduction
Rapid Brucella Ab Test kit is based on competitive immuno-chromatography technique to qualitatively detect Brucella Ab in bovine,goat,sheep and dog`s serum, plasma and whole blood. When testing, Brucella Ab in sample competition with antibody on T-line.The T line appears, means there is no Brucella Ab in the sample.
Contents
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1
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Brucella Test Device
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10 pcs
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2
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Capillary sampling tube
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10 pce
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3
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Dilution Buffer
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1 bottle
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4
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Instruction for use
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1 insert
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Procedure
Ⅰ Specimen: whole Blood or serum or plasma
1. Collected Blood 0.5-1mL, 3000 rev / min, centrifuged 3-5 minutes to separate the serum. Can also be 4 º C standing overnight, so that the natural precipitation of serum. Or direct test whole blood.
2. Remove the test device from the foil pouch, and place it on a flat and dry surface.
3. Take 5 μl (capillary black scale) serum or whole blood to the sample well, and then add a drop of buffer dilution.
4. Please wait 5 minutes.
5. Add two drops of buffer dilution.
6. At this time will see the wine red liquid flow through the observation window. Wait 10-15 minutes to determine the results, results will invalid after 15 minutes.
Ⅱ Specimen: Milk
1. Collect raw milk to ensure that the sample is not spoiled and not heated.
2. Remove the test card (if refrigerated, return it to room temperature and open the package) and place it flat on the table after opening.
3. Take 20ul raw milk, add the sample wells.
4. Please wait 5 minutes.
5. Add three drops of Buffer diluent to the sample well.
6. At this time will see the wine red liquid flow through the observation window. Wait 10-15 minutes to determine the results, results will invalid after 15 minutes.
■ Interpretation of the test
1） Negative：Both on T Line and C line being seen wine-red color reaction.
2） Positive：no color reaction on Test Line (T Line),only on Control Line(C Line) being seen wine-red color reaction.
3） Invalid：No color reaction on C Line.
packing
10T/kit.
Stability and Storage
Store at 2 - 30ºC and the shelf life is 24 months.
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