Nucleases are widely present in the laboratory environment and can interfere with many experiments.

 In particular, single stranded RNases are ubiquitous, hard to eliminate, and can rapidly degrade

 important samples used in microarray studies, real-time PCR, northern blots, or cDNA cloning.

 

IDT has developed reagents that allow for rapid, sensitive detection of RNases and DNases. These 

reagents are fluorescence-quenched oligonucleotide probes that emit a fluorescence signal only 

after nuclease degradation. The assay can be read visually or measured and quantified using 

fluorometry. Assays can be used qualitatively to test lab reagents, equipment, and supplies for 

nuclease contamination. Assays can be used quantitatively to study enzyme kinetics. Two 

oligonucleotide substrates are available: one designed to detect RNases and the second designed

 to detect DNases. The RNaseAlert® substrate employs a FAM™ reporter (Em 520 nm). The 

DNaseAlert™ substrate employs a HEX™ reporter (Em 555 nm).

 

 

RNaseAlert and DNaseAlert are available as:

·Single-use tubes (25 tubes with 0.05 nmole each)—ideal for rapid hand held visual assays

 at the research bench

·Bulk substrate (2 tubes with 2 nmole each)—for use in either visual assays or read in a fluorometer

·Kit—includes single use tubes, buffer, water, enzyme, and nuclease decontamination solution

RNaseAlert®

 

Kit Components:

·25 50 pmole Fluorescent Substrate tubes

·250 µL RNaseAlert® Buffer

·2 mL Nuclease-Free water

·50 µL RNase A

·50 mL Nuclease Decontamination Solution

·Kit quality guaranteed for six months from date received when stored at –20°C. 

 

 

DNaseAlert™

Kit Components:

·25 50 pmole Fluorescent Substrate tubes

·250 µL DNaseAlert™ Buffer

·2 mL Nuclease-Free water

·25 µL 2U/mL DNase 1 Enzyme

·50 mL Nuclease Decontamination Solution

·Kit quality guaranteed for six months from date received when stored at –20°C. 

About IDT

Integrated DNA Technologies (IDT) is a leader in manufacturing and developing products for the research and diagnostics life science market. IDT serves the areas of academic research, biotechnology, and pharmaceutical development. IDT was founded by Dr Joseph Walder in 1987. Since then, its development has been guided by an uncompromising approach to quality, a belief in the value of good service, and a determination to minimize consumer costs.

Serving over 80,000 life sciences researchers, IDT is widely recognized as the industry leader in custom oligonucleotides due to its capabilities in:

Analytical Sophistication—IDT pioneered the use of high throughput quality control (QC) methods and is the only oligonucleotide manufacturer to offer purity guarantees and 100% QC. Every oligonucleotide is analyzed by mass spectrometry and purified oligonucleotides receive further analysis by CE and HPLC.
Design Engineering—IDT maintains an engineering division dedicated to advancing synthesis, processing technology, and automation. An in-house machine shop provides rapid prototyping and custom part design/control.
Customer Support—IDT received over 100,000 calls last year with an average wait time of only 8 seconds.
Reagent and Input Control—IDT receives all solvents in large bulk containers, runs QC on all incoming materials, and performs bulk reagent formulation and functional QC on all reagents.
IDT has over 700 employees serving our worldwide customer base from headquarters in Coralville, Iowa, and facilities in San Diego, California; Leuven, Belgium; and Singapore.