Two oligonucleotide formulations for use as size/mass standards are available. Marker oligos have balanced base content, are purified and provided in equal mass amounts (10 µg of each oligo per tube) to normalize band intensity. (Note that the 10-mer binds dyes poorly and may appear more faint than the other bands.) Each tube contains enough marker for 25-100 loadings, depending on gel configuration and stain employed. Recommended loading mass varies with the precise stain used and dimensions of gel comb. For 1mm x 4-6mm comb dimensions, 0.1-0.2 µg/lane is recommended if using SybrGold™ stain (Molecular Probes). 0.2-0.4 µg/lane is recommended if using GelStar™ (Cambrex). For non‑fluorescent stains such as Stains‑All™ (Sigma-Aldrich), use 0.3-0.4 µg/lane. Ethidium Bromide staining works poorly with single-stranded nucleic acid and is not recommended.
10/60 Ladder
10, 15, 20, 25, 30, 40, 50 , 60 base marker oligos
20/100 Ladder
20, 30, 40, 50, 60, 70, 80, 90, 100 base marker oligos
bio-equip.cn About IDT
Integrated DNA Technologies (IDT) is a leader in manufacturing and developing products for the research and diagnostics life science market. IDT serves the areas of academic research, biotechnology, and pharmaceutical development. IDT was founded by Dr Joseph Walder in 1987. Since then, its development has been guided by an uncompromising approach to quality, a belief in the value of good service, and a determination to minimize consumer costs.
Serving over 80,000 life sciences researchers, IDT is widely recognized as the industry leader in custom oligonucleotides due to its capabilities in:
Analytical Sophistication—IDT pioneered the use of high throughput quality control (QC) methods and is the only oligonucleotide manufacturer to offer purity guarantees and 100% QC. Every oligonucleotide is analyzed by mass spectrometry and purified oligonucleotides receive further analysis by CE and HPLC.
Design Engineering—IDT maintains an engineering division dedicated to advancing synthesis, processing technology, and automation. An in-house machine shop provides rapid prototyping and custom part design/control.
Customer Support—IDT received over 100,000 calls last year with an average wait time of only 8 seconds.
Reagent and Input Control—IDT receives all solvents in large bulk containers, runs QC on all incoming materials, and performs bulk reagent formulation and functional QC on all reagents.
IDT has over 700 employees serving our worldwide customer base from headquarters in Coralville, Iowa, and facilities in San Diego, California; Leuven, Belgium; and Singapore.
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