While many researchers continue to store oligonucleotides in water, resuspension in a buffered solution such as TE is recommended. DNA oligonucleotides can be damaged by prolonged incubation or storage in even mildly acidic solutions; DNA dissolved in distilled water will often have a final pH <5.0 and is at risk for depurination. RNA will degrade in alkaline conditions. Further, single-stranded nucleic acids can easily be degraded by trace contamination with a variety of nucleases that are common contaminants in a normal laboratory environment. Any solution used to resuspend and store DNA or RNA should be nuclease-free. It is recommended that stock oligo solutions be made at high concentration and stored frozen. More dilute working solutions can be made from the stocks at intervals as needed.

 

IDTE (10 mM Tris, pH 7.5 or 8.0, 0.1 mM EDTA)

A 1x TE buffer for initial resuspension and storage of DNA oligos.

 

Nuclease Decontamination Solution

Many RNases and DNases are naturally found in the environment. Further, a variety of nucleases are employed in routine molecular biology methods and can accidentally contaminate lab surfaces at very high levels. In particular, RNases can be very difficult to eliminate and can cause damage to projects that require manipulations of intact RNA samples, such as functional genomics experiments, real-time quantitative PCR, microarrays, etc.

Nuclease Decontamination Solution irreversibly inactivates nucleases and can be applied to most lab surfaces to remove nuclease contamination. Just spray, rinse and let dry. Nuclease Decontamination Solution eliminates the need to bake glassware and can be applied to plastic surfaces which are difficult to sterilize.

Description	Part #	Pricing
250 mL Nuclease Decontamination Solution	11-05-01-01	$35.00 USD
6 x 250 mL Nuclease Decontamination Solution	11-05-03-01	$165.00 USD

Nuclease-free Water

For initial resuspension and storage of single-stranded RNA oligos. Also suitable for making dilute working solutions from stock oligos.

 

Description	Part #	Pricing
1 Liter Nuclease Free Water	11-05-01-04	$28.00 USD
10 x 2ml Nuclease Free Water	11-04-02-01	$16.00 USD
4 x 1 Liter Nuclease Free Water	11-05-02-02	$90.00 USD
300 mL Nuclease Free Water	11-05-01-14	$14.00 USD

Duplex Buffer (30 mM HEPES, pH 7.5, 100 mM Potassium Acetate)

For initial resuspension, annealing, and storage of duplex RNAi products.

 

Description	Part #	Pricing
1 Liter Nuclease Free Duplex Buffer	11-05-01-03	$30.00 USD
1 Liter Nuclease Free Water	11-05-01-04	$28.00 USD
10 x 2 ml Nuclease Free Duplex Buffer	11-01-03-01	$18.00 USD
4 x 1 Liter Nuclease Free Duplex Buffer	11-05-02-01	$95.00 USD
300 mL Nuclease Free Duplex Buffer	11-05-01-12	$18.00 USD

About IDT

Integrated DNA Technologies (IDT) is a leader in manufacturing and developing products for the research and diagnostics life science market. IDT serves the areas of academic research, biotechnology, and pharmaceutical development. IDT was founded by Dr Joseph Walder in 1987. Since then, its development has been guided by an uncompromising approach to quality, a belief in the value of good service, and a determination to minimize consumer costs.

Serving over 80,000 life sciences researchers, IDT is widely recognized as the industry leader in custom oligonucleotides due to its capabilities in:

Analytical Sophistication—IDT pioneered the use of high throughput quality control (QC) methods and is the only oligonucleotide manufacturer to offer purity guarantees and 100% QC. Every oligonucleotide is analyzed by mass spectrometry and purified oligonucleotides receive further analysis by CE and HPLC.
Design Engineering—IDT maintains an engineering division dedicated to advancing synthesis, processing technology, and automation. An in-house machine shop provides rapid prototyping and custom part design/control.
Customer Support—IDT received over 100,000 calls last year with an average wait time of only 8 seconds.
Reagent and Input Control—IDT receives all solvents in large bulk containers, runs QC on all incoming materials, and performs bulk reagent formulation and functional QC on all reagents.
IDT has over 700 employees serving our worldwide customer base from headquarters in Coralville, Iowa, and facilities in San Diego, California; Leuven, Belgium; and Singapore.