Ethylenediamine Alkoxylate Block Copolymer

•Average Molecular Weight: 18,000

•HLB: >24

S9 Surfactant Pluracare 1307 Prill / Tetronic 1307 Core Applications
(1) Stabilization of Reagent Components

•Targets: Enzymes, antibodies, antigens, fluorescent markers, etc.

•Mechanism:

•Forms micelles (low critical micelle concentration, ~0.001–0.01%) to encapsulate hydrophobic regions, reducing protein denaturation.

•Mitigates interfacial stress damage during lyophilization/reconstitution (synergizes with sugar-based protectants like trehalose).

•Applications:

•ELISA Reagents: Protects horseradish peroxidase (HRP) activity, preventing inactivation from freeze-thaw cycles.

•Chemiluminescence Reagents: Stabilizes luminescent markers (e.g., acridinium esters), reducing background signals.

(2) Anti-Interference (Reduction of Non-Specific Binding)

•Targets: Lipoproteins, cell debris, fibrinogen in serum/plasma.

•Mechanism:

•Hydrophobic PPO chains adsorb lipids, forming micelles to "trap" them and avoid non-specific binding with detection antibodies.

•PEO chains create a hydrophilic barrier, blocking protein adsorption on hydrophobic surfaces (e.g., microplates, magnetic beads).

•Typical Formulations:

•Immunoturbidimetric Reagents: 0.05–0.2% Tetronic 1307 + 0.1% signal enhancer BAP-1 to disperse chylomicrons in lipemic samples.

•Nucleic Acid Extraction Reagents: Utilizes weak cationic properties to bind impurity proteins, improving DNA purity.

(3) Nanomaterial Dispersion

•Targets: Gold nanoparticles (AuNPs), quantum dots, magnetic beads.

•Mechanism:

•PPO chains adsorb onto nanoparticle surfaces, while PEO chains extend outward to provide steric stabilization.

•Star-shaped structure enhances steric repulsion, preventing particle aggregation (superior to linear surfactants like Tween-20).

•Application Examples:

•Lateral Flow Strips: 0.01–0.05% Tetronic 1307 disperses gold-labeled antibodies, improving color uniformity on test lines.

•Homogeneous Immunoassays: Stabilizes fluorescent magnetic microspheres, reducing sedimentation.

(4) Lyoprotection and Reconstitution Optimization

•Function: Prevents inactivation of active ingredients during lyophilization and accelerates reconstitution.

•Mechanism:

•Forms a glassy matrix to minimize ice crystal-induced damage to protein structures.

•Facilitates rapid dispersion and reduces interfacial tension during reconstitution.

•Example Formulation:

•Lyophilized PCR Enzyme Mix: 1% trehalose + 0.1% Tetronic 1307 + 0.05% signal enhancer BAP-1.

 

Pluracare® and Tetronic® are trademarks of BASF.

 

S9 Surfactant_Surfactants_IVD materials_AccuSift

Shenzhen AccuSift Biotechnology Co., Ltd. (referred to as AccuSift) was established in January 2014. It is a technology-oriented enterprise specializing in the preparation of antigens and antibodies for IVD raw materials and food safety raw materials, as well as the development and application of biotechnology. The company has an advanced and complete production platform for single and polyclonal antibodies and has prepared a variety of small molecule hapten related to IVD raw materials, pesticides and veterinary drugs, antibiotics, mycotoxins, hormones, heavy metals, pigments, etc., and high-quality monoclonal antibodies. The company provides comprehensive antibody services, antigen design, synthesis, antibody preparation and labeling, and immunological experiments for domestic and foreign users. The company's antibody research and development department has high-quality talents, strong research and development capabilities and a quality assurance system, providing high-precision and high-stability product solutions for clinical diagnosis and food safety detection.

AccuSift adheres to the business philosophy of "integrity, innovation and progress", with the mission of "focusing on the source of precision and safeguarding the beauty of health", providing strong support for researchers in the fields of clinical diagnosis and food safety.