Biotinylated BTN1A1/Butyrophilin His&Avi Tag Protein, Human,specification,price,image-Bio-Equip in China

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Product Specification

Species	Human
Synonyms	Butyrophilin Subfamily 1 Member A1; BT; BTN
Accession	Q13410-1
Amino Acid Sequence	Ala26-Arg242 with His & Avi Tag at C-Terminus
Expression System	HEK293
Molecular Weight	33-40kDa (Reducing)
Purity	> 90 % as determined by SDS-PAGE & > 85 % as determined by SEC-HPLC
Endotoxin	<1EU/μg
Conjugation	Biotin
Tag	Avi Tag, His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, PH7.4, 5% trehalose
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.Sandstrom, A. et al. (2014) Immunity 40:490.

Background

BTN1A1, also known as BTN, is a member of the immunoglobulin superfamily and the major protein linked to milk fat droplets. It may function as a cell surface receptor. Its B30.2 domain binds to xanthine oxidoreductase (XOR), stabilizing XOR's association with the milk fat globule membrane and playing a key role in milk fat globule secretion. This binding is conserved among BTN1A1 orthologs but isn't shared by BTN2A1 or BTN3A1. The B30.2 domain in butyrophilins can sense changes in intracellular phospho-antigen (pAg) levels, and its binding to pAg triggers gamma δ T cell activation. Moreover, BTN1A1 regulates milk lipid content and droplet size, and it can inhibit the proliferation and metabolism of CD4 and CD8 T-cells activated by anti-CD3 antibodies, as well as their secretion of IL2 and IFNG.