PNGase F (N-Glycosidase F) is an asparagine amidase produced by Elizabethkingia miricola. PNGase F releases intact N-glycans by hydrolyzing the amide bonds of asparagine side chains (Asn-X-Ser/Thr, where X ≠ Pro), while converting Asn to aspartic acid (Asp). Unlike other glycosidases (such as Endo H), PNGase F can cleave all types of N-glycans (high-mannose, hybrid, and complex oligosaccharides), but it does not act on O-linked glycans or plant/insect N-glycans modified by core α-1,3-fucosylation. This enzyme is of significant importance in glycoprotein research, quality control in biopharmaceuticals, and proteomics. To enhance the convenience of experiments, we have developed the Fast PNGase F Kit, which simplifies the experimental procedures and reduces the reaction time to less than 20 minutes, thereby significantly improving experimental efficiency.

1. Two -Step deglycosylation

1). Add the following components to one tube on ice:

2). Denaturation: heating to 80℃ for 2 min

3). Deglycosylation: immediately after the reaction was completed, it was cooled on ice, and 1 μl of PNGase F was added and incubated at 50ºC for 10 minutes.

2. One -Step deglycosylation

1). Add the following components to one tube on ice:

2). Deglycosylation: 1 μl of PNGase F was added and incubated at 50ºC for 10 minutes.

Two-step enzyme digestion is recommended to ensure higher enzyme digestion efficiency. 

This product is for scientific research purposes only. 

For your safety and health, please wear a lab coat and disposable gloves.