Product Specification| Species | Human | | Synonyms | α-chymotrypsin A and B, alpha-chymotrypsin | | Expression System | E.coli | | Molecular Weight | 27 kDa (Reducing) | | Purity | ＞95% by SDS-PAGE and HPLC | | Tag | His Tag | | Physical Appearance | Lyophilized Powder | | Storage Buffer | 1 mM HCl, 2 mM CaCl2, pH 3.0 | | Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
| | Stability & Storage | Store at -25 ~ -15℃ for 2 years | | Reference | 1. Simon LM, Kotormán M, Garab G, Laczkó I. Structure and activity of alpha-chymotrypsin and trypsin in aqueous organic media. Biochem Biophys Res Commun. 2001 Feb 9;280(5):1367-71. PMID: 11162681.
2. Pham VT, Ewing E, Kaplan H, Choma C, Hefford MA. Glycation improves the thermostability of trypsin and chymotrypsin. Biotechnol Bioeng. 2008 Oct 15;101(3):452-9. PMID: 18470893. |
BackgroundChymotrypsin is a serine endoproteinase that specifically cleaves peptide bonds at the C-termini of Tyr, Phe, Trp and Leu. Met, Ala, Asp and Glu may be cleaved at a much lower rate. ComponentsRecombinant α-Chymotrypsin, human lyophilized powder ProtocolOptimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:
Add Recombinant α-Chymotrypsin, human, to a final protease: protein ratio of 1:200 to 1:20(w/w), and incubate sample for 2–18 hours at 25°C. The reaction may be stopped, if desired, by adding 0.5% trifluoroacetic acid. Unit DefinitionOne unit is defined as the amount of Chymotrypsin that will hydrolize 1.0µmol of BTEE per minute at pH 7.8 and 25°C. bio-equip.cn AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.
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