LAG-3/CD223 His Tag Protein, Rat,specification,price,image-Bio-Equip in China

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Product Specification

Species	Rat
Synonyms	Lymphocyte activation gene 3 protein，LAG3，Lymphocyte Activating 3，Lymphocyte-Activation Gene 3，CD223 Antigen，Protein FDC，CD223，LAG-3，FDC，sLAG-3
Accession	Q5BK54
Amino Acid Sequence	Gly24-Leu442 with His Tag at the C-Terminus
Expression System	HEK293
Molecular Weight	43-55kDa (Reducing)
Purity	＞95% by SDS-PAGE, ＞90% by SEC-HPLC &＞90% by RP-HPLC
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, PH7.4
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.Colin G. Graydon, Shifa Mohideen and Keith R.Fowke, LAG3’s Enigmatic Mechanism of Action. Frontiers in Immunology

Background

LAG3, a member of the immunoglobulin superfamily and a homolog of CD4, arose from a gene duplication event. Similar to CD4, LAG3 binds to MHC class II (MHCII), but it also interacts with FGL-1, α-synuclein fibrils (α-syn), galectin-3 (Gal-3), and lymph node sinusoidal endothelial cell C-type lectin (LSECtin). As an immune checkpoint, LAG3 inhibits the activation of its host cell, promoting a more suppressive immune response. On T cells, LAG3 reduces the production of cytokines and granzymes, as well as cell proliferation, while encouraging differentiation into T regulatory cells.