Apolipoprotein A-I/Apo-A1 His Tag Protein, Human,specification,price,image-Bio-Equip in China

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Product Specification

Species	Human
Synonyms	Alp-1; APOA1; Apo-AI; apoA-I; Apolipoprotein A1; Apolipoprotein AI; Apolipoprotein A-I; Brp-14; Ltw-1; Lvtw-1; MGC117399; Sep-1; Sep-2
Accession	P02647
Amino Acid Sequence	Arg19-Gln267 with His Tag at the C-Terminus
Expression System	HEK293
Molecular Weight	25-33kDa (Reducing)
Purity	＞95% by SDS-PAGE & ＞90% by RP-HPLC
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, PH7.4, 5% trehalose
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.Williams, D.L. et al. (2000) J. Biol. Chem. 275:18897. 2.Martinez, L.O. et al. (2003) Nature 421:75.

Background

Apolipoprotein A1 (ApoA1) is a 28 kDa glycoprotein and the primary protein component of high-density lipoprotein (HDL) particles. HDL particles are central to the reverse cholesterol transport process, moving cholesterol from peripheral tissues to the liver for excretion. ApoA1 plays a specific role in lipid metabolism. Chylomicrons, secreted from intestinal enterocytes, contain ApoA1, which is rapidly transferred to HDL in the bloodstream. This protein facilitates cholesterol efflux from tissues to the liver and serves as a cofactor for lecithin cholesterol acyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. ApoA1 binds to the scavenger receptor class B type I (SR-BI) and the beta chain of ATP synthase on hepatocytes. Hepatocytes then internalize these particles and transfer cholesterol into bile for excretion.