Sortase A,specification,price,image-Bio-Equip in China

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Product Specification

Species	Staphylococcus aureus
Synonyms	LPXTG specific sortase A, sortase, Srt A
Expression System	E.coli
Molecular Weight	25 kDa (Reducing)
Purity	＞98% by SDS-PAGE
Tag	No Tag
Physical Appearance	Liquid
Storage Buffer	50mM Tris-HCl, 100mM NaCl, 2.5mM KCl, pH 8.0, 0.04% Tween 20, 20%甘油
Stability & Storage	Store at -25 ~ -15℃ for 1 years，Avoid freeze / thaw cycle
Reference	1. Frankel B A, Kruger R G , Robinson D E ,et al. Staphylococcus aureus sortase transpeptidase SrtA: insight into the kinetic mechanism and evidence fora reverse protonation catalytic mechanism.[J].Biochemistry, 2005, 44(33):11188-11200. 2. Parthasarathy R, Subramanian S, Boder E T. Sortase A as a Novel Molecular "Stapler" for Sequence-Specific Protein Conjugation[J]. Bioconjugate Chemistry, 2007, 18(2):469-476.

Background

Sortase is a family of transpeptidases found in Gram-positive bacteria, which is divided into two subfamilies: Sortase A and Sortase B. Its main function is to catalyze the covalent attachment of cell surface proteins to the cell wall. Among them, Sortase A (SrtA) has been studied more extensively. Sortase A (SrtA) can specifically recognize proteins with a C-terminal conserved amino acid sequence LPXTG and an N-terminal polyglutamine (X is any amino acid except cysteine and tryptophan).

Components

0.5 ug/ul Sortase A in 50mM Tris-HCl, 100mM NaCl, 2.5mM KCl, 0.04% Tween 20，20%甘油, pH 8.0 @ 25°C
3×Reaction Buffer：100mM Tris-HCl、50mM CaCl2、20mM DTT, pH 7.5 @ 25°C

Protocol

1. Typical polypeptide reaction conditions are as follows:

Components	Volume	Final Concentration
Polypeptide 1 (1 mM)	5 µl	166 uM
Polypeptide 2 (1 mM)	5 µl	166 uM
Sortase A (0.5 ug/ul)	5 µl	-
3X Reaction Buffer	10 µl	1X
ddH2O	Up to 30µl	-

2. After preparation according to the above table, mixed centrifugation, reaction at 30℃ for 1-3h, and SDS-PAGE electrophoresis was performed after reaction.

Guidelines

Avoid freeze / thaw cycle

This product is for research use only.