Pfu DNA Polymerase,specification,price,image-Bio-Equip in China

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Product Specification

Species	Pyrococcus furiosus
Synonyms	DNA polymerase、 DNA polymerase B、Pfu polymerase、Pol I
Expression System	E.coli
Molecular Weight	90 kDa (Reducing)
Purity	＞95% by SDS-PAGE
Tag	His Tag
Storage Buffer	20 mM Tris-HCl, 0.1 mM EDTA, 0.1% Tween20, 0.1% triton X100, 1 mM DTT, 100 mM KCl, 50%Glycerol、pH 8.2 @ 25°C
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	[1] Picard Véronique, et al. "A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase." Nucleic Acids Research 22.13(1994):2587-91. [2] Yan, Wang , et al. "A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro." Nucleic Acids Research 3(2004):1197.

Background

Pfu DNA Polymerase is a thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90,000 daltons as estimated from the predicted amino acid sequence and exhibits 3´→5´ exonuclease (proofreading) activity. Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high fidelity.

Components

Storage Solution : 5 U/ul Pfu DNA Polymerase, 20 mM Tris-HCl, 0.1 mM EDTA, 0.1% Tween20, 0.1% triton X100, 1 mM DTT, 100 mM KCl, 50%Glycerol、pH 8.2 @ 25°C
10* Reaction Buffer: 200 mM Tris-HCl (pH 8.8) , 20 mM MgSO4, 100 mM KCl, 100 mM (NH4) 2SO4, 1% Triton X-100, 1 mg/ml nuclease-free BSA

Guidelines

Note: 1. It is critical to withhold Pfu DNA Polymerase until after the addition of dNTPs; otherwise, the proofreading activity of the polymerase may degrade the primers, resulting in nonspecific amplification and reduced product yield.

2. Assemble on ice.

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C