TPBG/5T4 Fc Chimera Protein, Mouse,specification,price,image-Bio-Equip in China

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Product Specification

Species	Mouse
Synonyms	TPBG,5T4, M6P1,5T4AG, WAIF1, 5T4 oncofetal antigen, Trophoblast glycoprotein,5T4 oncofetal trophoblast glycoprotein
Accession	Q9Z0L0
Amino Acid Sequence	Ser32-Ser361 with Human IgG1 Fc at C-Terminus
Expression System	HEK293
Molecular Weight	72-95kDa (Reducing)
Purity	＞95% by SDS-PAGE & SEC-HPLC
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	Human IgG1 Fc
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, PH7.4, 5% trehalose
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.Kagermeier-Schenk, B. et al. (2011) Dev. Cell. 21:1129.

Background

5T4, also known as Trophoblast glycoprotein and Wnt-activated inhibitory factor 1 (WAIF1), is a single-pass transmembrane protein which may function as an inhibitor of Wnt/beta-catenin signaling. It has been identified that TIP-2/GIPC, a common interacting protein involved in cancer, interacts with 5T4 through its PDZ domain. 5T4 inhibits Wnt/ beta -catenin signaling by binding to LRP6 and inhibit Wnt-induced LRP6 internalization into endocytic vesicles. Conversely, 5T4 can activate noncanonical Wnt signaling through DKK1. In addition to LRP6, in-house data indicates 5T4 can also interact with Frizzled8.