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Bst DNA Polymerase, Full length
Bst DNA Polymerase, Full length
Origin of place Singapore
Model UA070064-500U
Supplier ANT BIO PTE.LTD.
Price 100
Hits 5
Updated 8/27/2025
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Product Specification


SynonymsDNA polymerase I
Expression SystemE.coli
Molecular Weight

100kDa (Reducing)

Purity>95% by SDS-PAGE
TagHis Tag
Storage Buffer50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C
Stability & Storage

Store at -25 ~ -15℃ for 2 years

Reference

1. Genetic Analysis: Biomolecular Engineering, 1996, 12(5-6):185-195.
2. BioTechniques, 1991, 11(1):76-8, 80, 82-7.

Background

Bst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5´ → 3´ polymerase and double-strand specific 5´ → 3´ exonuclease activity, but lacks 3´ → 5´ exonuclease activity. It can be used to isothermal DNA amplification and Primer extension.

Components

50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C
10* Reaction Buffer: 200 mM Tris-HCl、100 mM (NH4)2SO4、100 mM KCl、20mM MgSO4、0.1% Tween® 20、pH 8.8@25°C
Magnesium Sulfate (MgSO4) Solution:100mM MgSO4

Protocol

Incubate the following reaction at 65°C for 30–60 minutes

Component

Final Concentration

10X Isothermal Amplification Buffer II

1X (contains 2 mM MgSO4)

MgSO4 (100 mM)

6 mM (8 mM total)

dNTP Mix (10 mM)

1.4 mM each

FIP/BIP Primers (25X)

1.6 µM

F3/B3 Primers (25X)

0.2 µM

LoopF/B Primers (25X)

0.4 µM

Bst  DNA Polymerase (8,000 U/ml)

320 U/ml

DNA or RNA Sample

> 10 copies or more

Nuclease-free Water

to 25 µl

Total Reaction Volume

25 µl

Guidelines

Bst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.

Reaction temperatures above 70°C are not recommended.

Cannot be used for thermal cycle sequencing or PCR.

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C
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