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Bst DNA Polymerase, Full length
Origin of place |
Singapore  |
Model |
UA070064-500U |
Supplier |
ANT BIO PTE.LTD. |
Price |
100 |
Hits |
5 |
Updated |
8/27/2025 |
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Product SpecificationSynonyms | DNA polymerase I | Expression System | E.coli | Molecular Weight | 100kDa (Reducing) | Purity | >95% by SDS-PAGE | Tag | His Tag | Storage Buffer | 50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C | Stability & Storage | Store at -25 ~ -15℃ for 2 years | Reference | 1. Genetic Analysis: Biomolecular Engineering, 1996, 12(5-6):185-195. 2. BioTechniques, 1991, 11(1):76-8, 80, 82-7.
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BackgroundBst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5´ → 3´ polymerase and double-strand specific 5´ → 3´ exonuclease activity, but lacks 3´ → 5´ exonuclease activity. It can be used to isothermal DNA amplification and Primer extension. Components50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C 10* Reaction Buffer: 200 mM Tris-HCl、100 mM (NH4)2SO4、100 mM KCl、20mM MgSO4、0.1% Tween® 20、pH 8.8@25°C Magnesium Sulfate (MgSO4) Solution:100mM MgSO4 ProtocolIncubate the following reaction at 65°C for 30–60 minutes Component | Final Concentration | 10X Isothermal Amplification Buffer II | 1X (contains 2 mM MgSO4) | MgSO4 (100 mM) | 6 mM (8 mM total) | dNTP Mix (10 mM) | 1.4 mM each | FIP/BIP Primers (25X) | 1.6 µM | F3/B3 Primers (25X) | 0.2 µM | LoopF/B Primers (25X) | 0.4 µM | Bst DNA Polymerase (8,000 U/ml) | 320 U/ml | DNA or RNA Sample | > 10 copies or more | Nuclease-free Water | to 25 µl | Total Reaction Volume | 25 µl |
GuidelinesBst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity. Reaction temperatures above 70°C are not recommended. Cannot be used for thermal cycle sequencing or PCR. Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C bio-equip.cn
AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.
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