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Bst DNA Polymerase, Large fragment
Origin of place |
Singapore  |
Model |
UA070065-800U |
Supplier |
ANT BIO PTE.LTD. |
Price |
48 |
Hits |
6 |
Updated |
8/27/2025 |
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Product SpecificationSynonyms | DNA polymerase I | Expression System | E.coli | Molecular Weight | 67kDa (Reducing) | Purity | >95% by SDS-PAGE | Tags & Cleavage sites | / | Tag | His Tag | Storage Buffer | 50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C | Stability & Storage | Store at -25 ~ -15℃ for 2 years | Reference | 1. Nucleic Acids Research, 2000, 28(12):E63. 2. Chemical Communications, 2014, 50(28):3747-3749. 3. Current Protocols in Molecular Biology, 2014(Suppl.105):15.14.1-15.14.14.
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BackgroundBst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity. It can be use to isothermal amplification (LAMP), DNA sequencing through high GC regions and Rapid Sequencing from nanogram amounts of DNA template. Components50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C 10* Reaction Buffer: 200 mM Tris-HCl、100 mM (NH4)2SO4、20mM MgSO4、100 mM KCl、1% Tween® 20、pH 8.8@25°C Magnesium Sulfate (MgSO4) Solution:100mM MgSO4 ProtocolIncubate the following reaction at 65°C for 30–60 minutes
Component | Final Concentration | 10X Isothermal Amplification Buffer II | 1X (contains 2 mM MgSO4) | MgSO4 (100 mM) | 6 mM (8 mM total) | dNTP Mix (10 mM) | 1.4 mM each | FIP/BIP Primers (25X) | 1.6 µM | F3/B3 Primers (25X) | 0.2 µM | LoopF/B Primers (25X) | 0.4 µM | Bst DNA Polymerase (8,000 U/ml) | 320 U/ml | DNA or RNA Sample | > 10 copies or more | Nuclease-free Water | to 25 µl | Total Reaction Volume | 25 µl |
Guidelines1.Bst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity. 2.100 µg/ml BSA or 0.1%Triton X-100 is required for long term storage. 3.Reaction temperatures above 70°C are not recommended. 4.Bst DNA Polymerase, Large Fragment cannot be used for thermal cycle sequencing or PCR Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C bio-equip.cn
AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.
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