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Bst DNA Polymerase, Large fragment
Bst DNA Polymerase, Large fragment
Origin of place Singapore
Model UA070065-800U
Supplier ANT BIO PTE.LTD.
Price 48
Hits 6
Updated 8/27/2025
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Product Specification


SynonymsDNA polymerase I
Expression SystemE.coli
Molecular Weight

67kDa (Reducing)

Purity>95% by SDS-PAGE
Tags & Cleavage sites/
TagHis Tag
Storage Buffer50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C
Stability & Storage

Store at -25 ~ -15℃ for 2 years

Reference

1. Nucleic Acids Research, 2000, 28(12):E63.
2. Chemical Communications, 2014, 50(28):3747-3749.
3. Current Protocols in Molecular Biology, 2014(Suppl.105):15.14.1-15.14.14.

Background

Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the
5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity. It can be use to isothermal amplification (LAMP), DNA sequencing through high GC regions and Rapid Sequencing from nanogram amounts of DNA template.

Components

50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C
10* Reaction Buffer: 200 mM Tris-HCl、100 mM (NH4)2SO4、20mM MgSO4、100 mM KCl、1% Tween® 20、pH 8.8@25°C
Magnesium Sulfate (MgSO4) Solution:100mM MgSO4

Protocol

Incubate the following reaction at 65°C for 30–60 minutes

Component

Final Concentration

10X Isothermal Amplification Buffer II

1X (contains 2 mM MgSO4)

MgSO4 (100 mM)

6 mM (8 mM total)

dNTP Mix (10 mM)

1.4 mM each

FIP/BIP Primers (25X)

1.6 µM

F3/B3 Primers (25X)

0.2 µM

LoopF/B Primers (25X)

0.4 µM

Bst  DNA Polymerase (8,000 U/ml)

320 U/ml

DNA or RNA Sample

> 10 copies or more

Nuclease-free Water

to 25 µl

Total Reaction Volume

25 µl



Guidelines

1.Bst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.

2.100 µg/ml BSA or 0.1%Triton X-100 is required for long term storage.

3.Reaction temperatures above 70°C are not recommended.

4.Bst DNA Polymerase, Large Fragment cannot be used for thermal cycle sequencing or PCR

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C
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