Endo S2,specification,price,image-Bio-Equip in China

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Product Specification

Species	Streptococcus pyogenes
Synonyms	Endo-beta-N-acetylglucosaminidase EndoS2、Endoglycosidase S2、EndoS2
Expression System	E.coli
Molecular Weight	93 kDa (Reducing)
Purity	＞95% by SDS-PAGE
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Liquid
Storage Buffer	20 mM Tris-HCl、50 mM NaCl、pH 7.5 @ 25°C
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	[1] Sjögren Jonathan, et al. "EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans." Glycobiology 10:1053-1063. [2] Jonathan Sjögren, et al. "EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and α1-acid glycoprotein. " Biochemical Journal 455.Pt 1(2013).

Background

Endo-S2 is an endo-n-acetylglucosaminase derived from streptococcus pyogenes that excises the glycosidic bond between the two innermost n-acetylglucosamines of the glycoprotein n-linking sugar chain. It is effective for high mannose type, hybrid type and double antenna complex type. Can be made for all human IgG, as well as mice, rats, monkey, goat, sheep, cattle and horse IgG antibody glycosyl.

Components

Storage Solution : 200U/ul Endo S2 、20 mM Tris-HCl, 50 mM NaCl. (pH 7.5 @ 25°C) 10*Reaction Buffer： 500 mM sodium acetate (pH 6.0 @ 25°C)

Protocol

1. Combine 100 µg of native IgG, 1 µl of 10*Reaction Buffer and H20 (if necessary) to make a 10 µl total reaction volume.

2. Add 1 µl EndoS2.

3. Incubate reaction at 37°C for 1 hour

Guidelines

Please avoid repeated freeze-thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10 µl.