Proteinase K,specification,price,image-Bio-Equip in China

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Product Specification

Synonyms	Proteinase K
Amino Acid Sequence
Expression System	PichiaPastoris
Molecular Weight	29 kD
Purity	＞95% by SDS-PAGE
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Liquid
Storage Buffer	20 mM Tris-HCl、1 mM CaCl2、50% Glycerol、pH 7.4 @ 25°C
Stability & Storage	Store at -25 ~ -15℃ for 1 years
Reference	[1] Kushnirov VV, Dergalev AA, Alexandrov AI. Proteinase K resistant cores of prions and amyloids. Prion. 2020 Dec;14(1):11-19. [2] Xu C , Battig A , Schartel B ,et al. Investigation of the Thermal Stability of Proteinase K for the Melt Processing of Poly(l-lactide)[J].Biomacromolecules, 2022, 23(11):4841-4850.

Background

Proteinase K is a powerful proteolytic enzyme with high specific activity and is a key reagent for DNA extraction. The enzyme is active in a wide pH range (4~12.5) and at high temperature (50~70°C), and is used for the isolation of plasmid or genomic DNA and RNA. In DNA extraction, the main function is to enzymatically dissociate the histones bound to nucleic acids, so that the DNA is free in the solution, and then extracted by different methods to remove impurities and collect the DNA. chelating agents such as EDTA or descaling agents such as SDS can not inactivate the enzyme.

Components

Storage Solution:20 mM Tris-HCl、1mM CaCl2、50% glycerol (pH 7.4 @ 25°C)

Protocol

Add the specified amount of Proteinase K storage solution according to the nucleic acid extraction instructions. Commonly used working concentrations are 50-100 µg/mL

Guidelines

Please avoid repeated freeze-thaw cycles.

Unit Definition

One unit is defined as amount of enzyme required to hydrolyze casein to produce 1µmoL L-tyrosine per minute under defined conditions.