GSPT1/ERF3A GST&His Protein, Human,specification,price,image-Bio-Equip in China

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Product Specification

Species	Human
Synonyms	ERF3A;eRF3aFLJ38048;ETF3A;eukaryotic peptide chain release factor GTP-binding subunit ERF3A;Eukaryotic peptide chain release factor subunit 3a;FLJ39067;G1 to S phase transition 1,551G9.2;G1 to S phase transition protein 1 homolog;GST1
Accession	P15170-1
Amino Acid Sequence	Phe294-Asp499, with C-terminal GST&His
Expression System	Baculovirus-InsectCells
Molecular Weight	51kDa (Reducing)
Purity	＞85% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	50mM Tris, 20mM GSH, 5%Glycerol, pH8.0
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	Cell. 2016 Nov 17;167(5):1229-1240.e15. Cell. 2006 Jun 16;125(6):1125-36.

Background

GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site.GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Binding of eRF1, eRF3, and GTP to pretermination complexes first induces a structural rearrangement that is manifested as a 2 nucleotide forward shift of the toeprint attributed to pretermination complexes that leads to GTP hydrolysis followed by rapid hydrolysis of peptidyl tRNA.