Product Specification| Species | Mouse | | Synonyms | IL-12a Protein, Mouse; IL-12b Protein, Mouse; IL-12p35 Protein, Mouse; IL-12p40 Protein, Mouse; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; CLMF p35; p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; NKSF1; NK cell stimulatory factor chain 1; interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; p35 Protein, Mouse; p40 Protein, Mouse | | Accession | P43432 、P43431 | | Amino Acid Sequence | p40: Met23-Ser335; p35: Arg23-Ala215, with C-terminal His Tag | | Expression System | CHO | | Molecular Weight | P40: 35.8 kDa (Reducing) P35: 22.5 kDa (Reducing)
| | Purity | ＞95% by SDS-PAGE and HPLC | | Endotoxin | <0.1EU/μg | | Conjugation | Unconjugated | | Tag | His Tag | | Physical Appearance | Lyophilized Powder | | Storage Buffer | PBS, pH7.4. | | Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
| | Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles. | | Reference | 1. Airoldi, I. et al. (2000) Journal of Immunology 165:6880. 2. Wolf, S.F. et al. (1991) Journal of Immunology 146:3074. 3. Gearing, D. et al. (1991) Cell 66:9. 4. Merberg, D. et al. (1992) Immunology Today 13:78. 5. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143.
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BackgroundIL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. bio-equip.cn AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.
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