VHL/Elongin-C/Elongin-B Complex Protein, Human,specification,price,image-Bio-Equip in China

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Product Specification

Species	Human
Antigen	ELOB/ELOC/VHL
Synonyms	Elongin B & Elongin C & VHL, ELOB & ELOC & VHL Complex, Elongin B & Elongin C & VHL Complex
Accession	Q15370、Q15369、P40337
Amino Acid Sequence	ELOB flag tag, Human, Asp2-Gln118, N-flag DYKDDDDKDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPDEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALCIEPFSSPPELPDVMKPQDSGSSANEQAVQ ELOC flag tag, Human, Asp2-Cys112, N-flag DYKDDDDKDGEEKTYGGCEGPDAMYVKLISSDGHEFIVKREHALTSGTIKAMLSGPGQFAENETNEVNFREIPSHVLSKVCMYFTYKVRYTNSSTEIPEFPIAPEIALELLMAANFLDC VHL His tag, Human, Pro2-Asp213, N-His HHHHHHHHHHGGGSGGGSPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD
Expression System	Baculovirus-InsectCells
Molecular Weight	14 kDa (ElOB) and 13.3 kDa (ElOC) and 25.9 kDa (VHL)
Purity	>95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	40mM Tris, 200mM NaCl, 2.2mM KCl, 20% glycerol, 3mM DTT, pH8.0
Reconstitution	N.A
Stability & Storage	12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.
Reference	1.Cell Chem Biol. 2023 Jul 20;30(7):766-779.e11. Epub 2023 Jun 23. 2.bioRxiv. 2023 May 24;2023.05.22.541439. PreprintOkumura F., et al. (2012) Front. Oncol. 3.Mod Pathol. 2023 Apr 23;36(8):100194. Online ahead of print.

Background

ELOB (elongin B) is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. ELOB and ELOC(elongin C) form a heterodimer that serves as the regulatory subunit for the Elongin complex-a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites. The ELOB/ELOC complex also binds to the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. The VHL(von Hippel-Lindau disease tumor suppressor) binds to ELOB and ELOC and thereby inhibits transcription elongation.