EphA5 His Tag Protein, Human,specification,price,image-Bio-Equip in China

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Product Specification

Species	Human
Synonyms	EHK-1, EK7, BSK, EHK1, HEK7, TYRO4, Rek7, TYRO4HEK7CEK7
Accession	P54756
Amino Acid Sequence	Pro25-Pro573, with C-terminal 10*His PASLAGCYSAPRRAPLWTCLLLCAALRTLLASPSNEVNLLDSRTVMGDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCNSLPGGLGTCKETFNMYYFESDDQNGRNIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSKKGFYLAFQDVGACIALVSVRVYYKKCPSVVRHLAVFPDTITGADSSQLLEVSGSCVNHSVTDEPPKMHCSAEGEWLVPIGKCMCKAGYEEKNGTCQVCRPGFFKASPHIQSCGKCPPHSYTHEEASTSCVCEKDYFRRESDPPTMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGRKDVSYYIACKKCNSHAGVCEECGGHVRYLPRQSGLKNTSVMMVDLLAHTNYTFEIEAVNGVSDLSPGARQYVSVNVTTNQAAPSPVTNVKKGKIAKNSISLSWQEPDRPNGIILEYEIKYFEKDQETSYTIIKSKETTITAEGLKPASVYVFQIRARTAAGYGVFSRRFEFETTPVFAASSDQSQIPGGGSGGGSHHHHHHHHHH
Expression System	HEK293
Molecular Weight	72-85kDa
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1. CHENGYING LIU, YAN HE, XIAO FENG, et al. Expression of EPHA5 in lung adenocarcinoma is associated with lymph node metastasis and EGFR mutation[J]. 2022,130(6):338-345.

Background

EPHA5 is a member of the Eph family of tyrosine kinase receptors, which affect carcinogenesis. High expression of EPHA5 was found in NCI‐H460 and H1299 cells, while low expression was observed in A549 and SPC‐A1 cells. EPHA5 was knocked down in NCI‐H460 and H1299 lung cancer cell lines using siRNAs. EPHA5 receptor is differently expressed in lung adenocarcinoma tissues, in which positive and negative expression of EPHA5 was found in 58.1% and 41.9% of tissues, respectively.EPHA5 receptor plays a role in tumor promotion in lung adenocarcinoma and it is a potential target for lung adenocarcinoma treatment.