Luciferase Ⅰ,specification,price,image-Bio-Equip in China

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Product Specification

Species	Photinus pyralis
Synonyms	N.A
Amino Acid Sequence	N.A
Expression System	E.coli
Molecular Weight	62kDa
Purity	>95% by SDS-PAGE
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Liquid
Storage Buffer	25 mM Tris-acetate (pH7.8),1 mM EDTA, 1 mM DTT, 50% glycerol, 0.2 M ammonium sulfate
Reconstitution	N.A
Stability & Storage	12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.
Reference	1.Mcelroy H H S D .THE COLORS OF FIREFLY BIOLUMINESCENCE: ENZYME CONFIGURATION AND SPECIES SPECIFICITY[J].Proceedings of the National Academy of Sciences of the United States of America, 1964, 52(1):75-81. 2.Conti E , Franks N P , Brick P .Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes[J].Structure, 1996, 4(3):287.

Background

Luciferase is a general term for enzymes that produce biofluorescence in nature. Luciferase can catalyze the oxidation of luciferin to oxyluciferin. In the process of luciferin oxidation, biofluorescence is emitted. The biofluorescence released during the oxidation of luciferin can then be measured by a fluorometer. The Luciferase came from the firefly Photinus pyralis (Ppy) catalyzes a two-step reaction that results in the oxidation of D-luciferin accompanied by emission of yellow−green light with a peak at 560 nm. However, wild-luciferase activity is inhibited by sodium chloride, Therefore, the amino acid mutation we performed improved the inhibition of sodium chloride and was eventually named Luciferase Ⅰ.

Unit Definition

One unit of Luciferase is defined as the chemiluminescence module of PerkinElmer VICTROR Nivo was used to record the luminescence reading output within 5 minutes, namely the luminescence quantity CPS.