GPC3/Glypican 3 His Tag Protein, Mouse,specification,price,image-Bio-Equip in China

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Product Specification

Species	Mouse
Synonyms	GPC3, OCI5, Glypican-3, GTR2-2, MXR7, DGSX, SDYS
Accession	Q8CFZ4
Amino Acid Sequence	Gln25-Met557, with C-terminal 10*His QPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQMMNPGLPESVLDINECLRGARRDLKVFGSFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNGGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKILKVAHVEHEETLSSRRRELIQKLKSFINFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSVPKGKVLDKSLDEEGLESGDCGDDEDECIGSSGDGMVKVKNQLRFLAELAYDLDVDDAPGNKQHGNQKDNEITTSHSVGNMGGGSGGGSHHHHHHHHHH
Expression System	HEK293
Molecular Weight	72-130kDa
Purity	>95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1、Kandil D H. et al. (2009) Glypican-3: a novel diagnostic marker for hepatocellular carcinoma and more. Adv Anat Pathol. 16(2): 125-129. 2、Maeda D. et al. (2009) Glypican-3 expression in clear cell adenocarcinoma of the ovary. Mod Pathol. 22(6): 824-832.

Background

Glypican-3 (GPC3) belongs to a six-member family of membrane-bound heparan sulfate proteoglycans with a gene that is localized to Xq26.2. GPC3 belongs to a family of Glypicans are heparan sulfate proteoglycans that are bound to the cell surface by glycosyl phosphatidyli nositol. While six members of the glypican family are known in mammals, our study focused on glypican 3(GPC3). Loss-of-function mutations of GPC3 result in the Simpson- Golabi-Behmel syndrome, an X-linked disorder characterized by pre- and postnatal liver and other organ overgrowth. GPC3 is overexpressed in human hepatocellular carcinoma; however, its role in normal liver regeneration and hepatocyte proliferation is unknown. GPC3 is located on the X chromosome, and is highly expressed during embryogenesis and organogenesis. In the adult, on the other hand, GPC3 can only be detected in a limited number of tissues, including the lung, ovaries, mammary epithelium and mesothelium.