Nectin-3/CD113 His Tag Protein, Human,specification,price,image-Bio-Equip in China

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Product Specification

Species	Human
Antigen	Nectin-3/CD113
Synonyms	CD113 antigen; CD113; CDw113FLJ90624; DKFZp566B0846; nectin 3; Nectin3; Nectin-3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; PPR3; PRR3CD113; PVRL3; PVRR3
Accession	Q9NQS3
Amino Acid Sequence	Gly58-Asp400, with C-terminal 8*His GPIIVEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSSQTVAVHHPQYGFSVQGEYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAICIAATGKPVAHIDWEGDLGEMESTTTSFPNETATIISQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDIQYAPEVSVTGYDGNWFVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTFNYSGVYICKVTNSLGQRSDQKVIYISDPPTTTTLQPTIQWHPSTADIEDLATEPKKLPFPLSTLATIKDDGGGSHHHHHHHH
Expression System	HEK293
Molecular Weight	65-85kDa (Reducing)
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.Takai, Y. and H. Nakanishi (2003) J. Cell Sci.116:17. 2.Miyoshi, J. and Y. Takai (2007) Am. J. Nephrol.27:590. 3.Sakisaka, T. et al. (2007) Curr. Opin. Cell Biol.19:593. 4.Rikitake, Y. and Y. Takai (2008) Cell. Mol. LifeSci. 65:253.

Background

Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. The Nectin family has at least four members (Nectin-1 - 4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma ), and three extracellular Ig-domains. Nectin-3 (also named PRR3, CD113 and PVRL3) is an 83 kDa, type I TM glycoprotein. NECTIN3 is intended to initiate cell-cell adhesion to stimulate cell attachment and to allow subsequent formation of JAM-and cadherin-based intercellular junctions. NECTIN3 induces endocytosis-mediated down-regulation of PVR from the cell surface, which results in reduction of cell movement & proliferation. Following Nectin-3 activity adds strength to the junction through trans-interaction with various molecules.