Carbonic Anhydrase IX/CA9 His Tag Protein, Human,specification,price,image-Bio-Equip in China

Product Detail
Company Profile

Product Specification

Species	Human
Antigen	Carbonic Anhydrase IX/CA9
Synonyms	Carbonate dehydratase IX, Carbonic anhydrase IX (CA-IX; CAIX), Membrane antigen MN, P54/58N
Accession	Q16790
Amino Acid Sequence	Gln38-Asp414, with C-terminal 8*His QRLPRMQEDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNSCLAAGDGGGSHHHHHHHH
Expression System	HEK293
Molecular Weight	51-54kDa
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1. Supuran CT. Carbonic anhydrases: novel therapeutic applications for inhibitors and activators. Nat Rev Drug Discov 2008;7:168-81. 2. McDonald PC, Swayampakula M, Dedhar S.. Coordinated regulation of metabolic transporters and migration/invasion by carbonic anhydrase IX. Metabolites 2018;8:E20.

Background

The CAIX works as a dimeric molecule composed of two monomeric proteins liked with a disulfide bond between cysteine residues of the two CAIX monomers, while the structure of CAXII is stabilized by 19 hydrogen-bonded interactions in the dimer interface. Carbonic anhydrases IX and CAXII (CAIX/CAXII) are transmembrane zinc metalloproteins that catalyze a very basic but crucial physiological reaction: the conversion of carbon dioxide into bicarbonate with a release of the proton. CAIX functions with bicarbonate transporters (including electroneutral bicarbonate transporter NBCn1 (SLC4A7)) to bring in HCO3- produced in a CAIX-mediated hydration reaction of CO2, and amino acid transporters (including the large neutral amino acid transporter 1 (LAT1) and CD98 heavy chain (CD98hc)) to transport in essential amino acids and glutamine (via interaction with glutamine transporter SLC1A5 and sodium-coupled neutral amino acid transporter 2 (SNAT2) facilitating the uptake of amino acid by SLC1A5), which cancer cells employ as an alternative metabolic energy source and biosynthetic precursor. CAIX also creates unique interactions with collagen- and laminin-binding integrins at the leading edge of migrating cells.