IdeZ Protease (Tag Free),specification,price,image-Bio-Equip in China

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Product Specification

Species	Streptococcus equi subsp. Zooepidemicus
Synonyms	IdeZ
Expression System	E.coli
Molecular Weight	36kDa (Reducing)
Purity	＞95% by SDS-PAGE
Conjugation	Unconjugated
Tag	No Tag
Physical Appearance	Liquid
Storage Buffer	Tris-HC, pH7.5.
Stability & Storage	12 months from date of receipt, -20 to -70 °C as supplied; 1 week, 2 to 8 °C under sterile conditions; Please avoid repeated freeze-thaw cycles.
Reference	[1] Shi S, Mcleod B, Magnelli P, et al. The Utility of IdeZ Protease (Tag Free) in Glycan Profiling of Therapeutic Antibodies[J]. Glycobiology, 2016(12):26. [2] Cheng-Wei H, JieP, Yu-Qing O ,et al. Characteristics of IgG degradation enzyme IdeZ in Streptococcus equi ssp. zooepidimicus[J].Chinese Journal of Preventive Veterinary Medicine, 2018..

Background

IdeZ Protease secreted by the Streptococcus equi subspecies zooepidemicus. This product is an engineered recombinant protease over expressed in Escherichia coli. It can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and produce F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs.

Components

20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5

Protocol

1. Add the desired amount of 5mg IgG in digestion buffer or other compatible buffer*.

2. Add IdeZ Protease (Tag Free)-1 to the reaction system:

• Add 1 unit of IdeZ Protease (Tag Free)-1 per 1µg of IgG to be digested.

• For example, add 5µl (200 units) of reconstituted IdeZ to digest 200µg of IgG.

3. Incubate sample at 37°C for 30–60 minutes

Guidelines

Please avoid repeated freeze-thaw cycles.

Unit Definition

One unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C