Leptin R/CD295 His Tag Protein, Human,specification,price,image-Bio-Equip in China

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Product Specification

Species	Human
Synonyms	LEP-R, HuB219, OB receptor (OB-R), CD295
Accession	P48357
Amino Acid Sequence	Phe22-Asp839, with C-terminal 10*His FNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPKFNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVFQQIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLEDSPLVPQKGSFQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVIFQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQVKYSENSTTVIREADKIVSATSLLVDSILPGSSYEVQVRGKRLDGPGIWSDWSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMNLAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHHRYAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMKNDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINSIGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMYFIIEWKNLNEDGEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGVGKPKIINSFTQDDIEKHQSDGGGSGGGSHHHHHHHHHH
Expression System	HEK293
Molecular Weight	100-120kDa
Purity	>95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1. Bj?rbaek C, Uotani S, da Silva B, Flier JS. Divergent signaling capacities of the long and short isoforms of the leptin receptor. J Biol Chem. 1997;272:32686-32695.

Background

Leptin cellular actions are mediated by leptin receptors (OB-Rs, Leptin R), which are membrane-anchored molecules binding to leptin and transducing its intracellular signal. Leptin R is encoded by the db gene. Leptin R is a member of cytokine receptor family that including various interleukins, interferon-γ, erythropoietin, growth hormone and prolactin. Proteolytic cleavage of transmembrane Leptin R produces a soluble leptin binding protein called soluble leptin receptor (sOB-R), which constitutes the major binding protein of circulating leptin in human plasma. The sOB-R exerts high affinity in binding leptin, antagonizing the membrane leptin receptors and, functionally, inactivating leptin. By binding circulating leptin, sOB-R modulates the bioavailability and efficacy of leptin, exerting a predominantly inhibitory effect on leptin signal transduction; thus, altering the sensitivity of target tissues to leptin actions and producing a state called leptin resistance in analogy to in sulin resistance. Genetic deficiency of either leptin or Leptin R causes severe obesity and obesity-associated diabetes.