Biotinylated Fc γ RIIIa/CD16a(V176) Avi&His Tag Protein, Human,specification,price,image-Bio-Equip in China

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Product Specification

Species	Human
Synonyms	CD16-II, CD16a antigen, FcR-10
Accession	P08637
Amino Acid Sequence	Gly17-Gln208, with C-terminal Avi and His Tag
Expression System	HEK293
Molecular Weight	40-45kDa (Reducing)
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	Avi Tag, His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4.
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1. Nimmerjahn, F. and J.V. Ravetch: Fcgamma receptors: old friends and new family members , Immunity Jan;24(1):19-28(2006). 2. Ravetch, J.V. and B. Perussia : Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions , J. Exp. Med. 170:481(1989).

Background

Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes. In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. A single nucleotide polymorphism creates high binding (V176) and low binding (F176) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.