PEAR1 His Tag Protein, Human,specification,price,image-Bio-Equip in China

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Product Specification

Species	Human
Synonyms	Jedi, PEAR1
Accession	Q5VY43
Amino Acid Sequence	Leu21-Ser754, with C-terminal His Tag
Expression System	CHO
Molecular Weight	80-100kDa (Reducing)
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4.
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.Nanda, N. et al. (2005) J. Biol. Chem. 280:24680. 2.Sun, Y. et al. (2015) Mol Cell Proteomics. 14(5): 1265-1274. 3.Elenbaas, J. et al. (2023) Nat Commun. 14: 850.

Background

Platelet endothelial aggregation receptor 1 (PEAR1) is a 150 kDa type I transmembrane protein and member of the MEGF family of proteins. Human PEAR1 is synthesized as a 1037 amino acid (aa) precursor that contains a 20 aa signal sequence, a 735 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 261 aa cytoplasmic region. Mature human PEAR1 is 84% aa identical to mature mouse PEAR1. PEAR1 is most highly expressed in platelets and endothelial cells. High affinity immunoglobulin E receptor subunit alpha (Fc epsilon R1 alpha ) has been identified as an activating platelet endothelium aggregation receptor 1 (PEAR1) ligand. Inherited PEAR1 variations that alter expression or function of the platelet signaling molecule could modify agonist-induced aggregation in native platelets.