DNase I (Deoxyribonuclease I), can digest single or double-stranded DNA to produce mono deoxynucleotides or single or double-stranded oligo deoxynucleotides, its optimal working pH range is 7-8. DNase I activity is dependent on Ca2+ and can be activated by other bivalent metal ions such as Mg2+, Mn2+, Zn2+, etc. In the presence of Mg2+, the enzyme can randomly recognize and cut any site on any strand of DNA. In the presence of Mn2+, two strands of DNA can be cut at the same site to form sticky ends with flat ends or 1-2 nucleotides protruding.

Storage Solution:  2 U/ul DnaseⅠ、10mM Tris-Hcl、2mM  CaCl2、50%Glycerol (pH7.6, 25℃)

10*Reaction Buffer: 100mM Tris-Hcl、25mM  MgCl2、5mM  CaCl2 (pH7.6, 25℃)

This step is suitable for linearization of 1 μg DNA (≥100 nt) and can be scaled up according to experimental needs.

1）Add the following components in sequence

2）Incubate at 37°C 1 h.

1. EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation 2. Please avoid repeated freeze-thaw cycles