Product Specification| Species | Yeast | | Synonyms | Inorganic pyrophosphatase、PPase | | Concentration | 0.1U/μl | | Expression System | E.coli | | Molecular Weight | 33.5 kD | | Conjugation | Unconjugated | | Tag | His Tag | | Physical Appearance | Liquid | | Storage Buffer | 20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA 50% Glycerol (pH 8.0 @ 25°C) | | Stability & Storage |
Store at -25 ~ -15℃
for 2 years
| | Reference | [1] Lahti R , Pitk Ranta
T , Valve E ,et al. Cloning and characterization of the gene
encoding inorganic pyrophosphatase of Escherichia coli K-12.[J].Journal of
Bacteriology, 1988, 170(12):5901-7
[2] Satoh T , Watanabe M ,
Nogi S I ,et al. Molecular Cloning, Expression, and Site-Directed
Mutagenesis of Inorganic Pyrophosphatase from Thermus thermophilus HB8[J]. Journal
of Biochemistry, 1998, 124(1):79-88.
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Background
Pyrophosphatase, Inorganic is a yeast derived
inorganic pyrophosphatase (PPase) expressed by Escherichia coli, which is an
enzyme that catalyzes the conversion of one molecule of pyrophosphate to two
molecules of phosphate ions, causing the inorganic pyrophosphate hydrolysis to
form phosphates. In molecular biology, it can be used to increase the
production of RNA in vitro transcription reactions.
Components
Storage Solution: 0.1U/μl Pyrophosphatase, Inorganic、 20
mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA 50%
Glycerol (pH 8.0 @ 25°C)
Protocol
Use 1-3 units per ml
in a high yield in vitro RNA synthesis reaction
Unit DefinitionOne unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2 and 2 mM PPi). bio-equip.cn AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.
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