Murine RNase Inhibitor,specification,price,image-Bio-Equip in China

Product Detail
Company Profile

Product Specification

Species	Rat
Synonyms	Ribonuclease inhibitor
Expression System	E.coli
Molecular Weight	50 kDa (Reducing)
Purity	＞95% by SDS-PAGE
Conjugation	Unconjugated
Tag	No Tag
Storage Buffer	20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol (pH 7.6 @ 25℃)
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	[1] Wanhua, Guo, and et al. High level soluble production of functional ribonuclease inhibitor in Escherichia coli by fusing it to soluble partners[J]. Protein Expression & Purification, 2011. [2] Chen, C. Z. , and R. Shapiro . "Site-specific mutagenesis reveals differences in the structural bases for tight binding of RNase inhibitor to angiogenin and RNase A. " Proceedings of the National Academy of Sciences 94.5(1997):p. 1761-1766.

Background

Murine RNase inhibitor is expressed in soluble form in E. coli and capable of inhibiting a wide range of RNases (RNase A, B, C). Murine RNase inhibitor was tested by RT-PCR and RT-qPCR, and was compatible with various commercial reverse transcriptase’s and various DNA polymerases. Compared with human RNase inhibitor, murine RNase inhibitor does not contain two cysteines that are very sensitive to oxidation, so it has higher antioxidant activity and is more suitable for high DTT sensitive experiments (such as qPCR).

Components

Storage Solution: 40 U/µL Murine RNase Inhibitor, 20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol (pH 7.6 @ 25℃)

Protocol

Add inhibitor to achieve final concentration of 1 U/μL in your reaction.
During assembly of a reaction, RNase Inhibitor should be added before other components that are a possible source of RNase contamination.

Guidelines

Please avoid repeated freeze-thaw cycles.

Unit Definition

One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.