Endo S,specification,price,image-Bio-Equip in China

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Product Specification

Species	Streptococcus pyogenes
Synonyms	Endo-beta-N-acetylglucosaminidase EndoS, Endoglycosidase S, EndoS
Expression System	E.coli
Molecular Weight	109 kDa (Reducing)
Purity	＞95% by SDS-PAGE
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Liquid
Storage Buffer	20 mM Tris-HCl、50 mM NaCl、5 mM EDTA（pH 7.5 @ 25°C）
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	[1] Collin,M.EndoS, a novel secreted protein from Streptococcus pyogenes with endoglycosidase activity on human IgG[J].Embo Journal, 2014, 20(12):3046-3055. [2] Beatriz,Trastoy,Joseph,et al.Liquid–liquid diffusion crystallization improves the X-ray diffraction of EndoS, an endo-β-N-acetylglucosaminidase from Streptococcus pyogenes with activity on human IgG[J].Acta Crystallographica, 2013.

Background

Anenzyme expressed by Streptococcus pyogenes capable of releasing the terminal sialic acid residues from glycoproteins such as immunoglobulins.Specifically catalyzes the hydrolysis of the beta-1,4 linkage between the first two N-acetylglucosamine residues of the complex-type N-linked glycan located on 'Asn-297' of the Fc region of IgG antibodies (IGHG1, IGHG2, IGHG3 or IGHG4), thereby preventing interaction between IgGs and Fc receptors and ability to activate the complement pathway. Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG.

Components

Storage Solution : 200U/ul Endo S、20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA. (pH 7.5 @ 25°C)

10*Reaction Buffer：50mM CaCl2、500 mM sodium acetate (pH 5.5 @ 25°C)

Protocol

1. Combine 100 µg of native IgG, 1 µl of 10*Reaction Buffer and H20 (if necessary) to make a 10 µl total reaction volume.

2. Add 1 µl Endo S.

3. Incubate reaction at 37°C for 1 hour

Unit Definition

One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10µl.