The VSV-G glycoprotein is a trimeric protein that forms spikes on the surface of the vesicular stomatitis virus, contributing to its structural integrity and functionality. Each monomer within the trimer is composed of a single polypeptide chain, which folds into distinct domains that facilitate its role in viral processes. In addition to its structural roles, the VSV-G glycoprotein is involved in immune evasion. It can modulate host immune responses, allowing the virus to persist and replicate within the host. The VSV-G glycoprotein has emerged as a tool in the field of gene therapy, largely due to its ability to facilitate the delivery of genetic material into target cells.