CD300g/Nepmucin/CLM9 His Tag Protein, Human,specification,price,image-Bio-Equip in China

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Product Specification

Species	Human
Synonyms	CMRF35-Like Molecule 9; CLM-9; CD300 Antigen-Like Family Member G; Triggering Receptor Expressed on Myeloid Cells 4; TREM-4; CD300g; CD300LG; CLM9; TREM4
Accession	Q6UXG3-1
Amino Acid Sequence	Leu19-Arg247, with C-terminal His
Expression System	HEK293
Molecular Weight	35-72kDa (Reducing)
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4.
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.Umemoto, E. et al. (2013) PloS One. 8:e83681. 2.Umemoto, E. et al. (2006) J. Exp. Med. 203:1603. 3.Takatsu H, et al. (2006) Biochem. Biophys. Res. Commun. 348:183.

Background

Nepmucin, also known as CMRF35-like molecule 9 (CLM-9), TREM-4 and CD300g, is a 75-95 kDa type I transmembrane O-glycosylated member of the CD300 family of molecules. Nepmucin plays a critical role in promoting lymphocyte transendothelial migration of high endothelial veins and mediates lymphocyte adhesion with endothelial cells by its Ig domain, which is independent of LFA-1 or VLA-4 adhesion pathway. Glycosylated nepmucin with MECA-79 epitope associate with L-selectin to mediate L-selectin-dependent lymphocyte rolling by its mucin-like domain. Expression of nepmucin is down-regulated in tumours and tumour-draining lymph nodes, indicating that the expression of nepmucin is negatively regulated by locally produced signals under these circumstances. Nepmucin induction can significantly improve the killing activity of CIK cells.Receptor which may mediate L-selectin-dependent lymphocyte rollings. Binds SELL in a calcium dependent manner.