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Gene function

gene function

Discovering how a gene functions requires the right tools. Guide-it CRISPR/Cas9 products provide a streamlined gene editing workflow to generate knockouts and knockins. Inducible expression technologies permit the tightest control over gene expression, and fluorescent proteins directly track and visualize cellular events. Transfection or viral transduction allow efficient delivery of a gene to target cells. iDimerize technology can be used to control the interactions or location of the expressed protein.

Innovative technology for delivery of CRISPR/Cas9 complexes:

Guide-it CRISPR/Cas9 Gesicle Production System

Production of cell-derived nanovesicles, called gesicles, containing active Cas9 protein complexed with sgRNA specific to a gene of interest for gene editing in target cells.

Products

T-cell transduction and culture

Tet-inducible expression systems

ProteoTuner protein control systems

iDimerize inducible protein interaction systems

Transfection reagents

Mammalian expression plasmids

Control protein interactions using dimerizer ligands such as AP20187 and AP21967

Instant control of protein-protein interactions in mammalian cells

Every process in the cell is regulated by a complex network of protein-protein interactions. iDimerize technology allows you to specifically, rapidly, and reversibly control the interaction between two proteins simply by adding or removing a small molecule ligand from the culture medium.