This product is intended for mycobacteria isolation and culture.
The product is a new pattern L-J medium used to culture mycobacterium. The solid part consists of all the improved ingredients of L-J medium common to both domestic and foreign professionals; The liquid part consists of basic medium, growth promoting agent, antibacterial agent, and chromogenic agent. Typical colony can form on the slant of the culture medium, as with chromogenic agent, pink or red particles will appears in the liquid part.
Solid culture medium, liquid culture medium, sterile diluent, antibacterial agent, package insert
· Sealed and stored at 2-8°C in a cool and dry place.
· The validity duration is 6 months
· Use immediately after unsealing.
· Do not freeze.
· Use immediately once antibacterial agent is dissolved, and it can be stored at 2-8°C for 72h and at -20°C 6 months only after dissolution.
· Human sputum as specimen for the test, which should be digested through alkaline treatment if contaminated.
· Best result will be likely to obtain with first mouthful of sputum spited out of deep lung in the morning.
· Timely detection is recommended after specimen collection, otherwise store at (2-8°C) in the fridge and finish the test within 72 hours.
· Pretreatment of the specimen: alkali treatment and centrifugation
· Take out antibacterial agent, open the cap, and shake up after adding the sterile diluent.
· Suck up 100ul of mixed antibacterial agent, then add into the liquid culture medium and shake up.
· Add the mixed culture medium into the solid culture medium slant.
· Inoculate 0.1ml of already treated specimen onto the slant of the solid culture medium; Do the same thing with the second biphasic L-J medium as the control test.
· Tilt the tube to let the liquid to submerge the entire surface of solid medium, keep the position at 37°C in incubator for 48h, and erect the tube to continue culture.
· Positive: Detection on positive control material
· Negative: Detection on negative control material
bio-equip.cn Zhuhai Encode Medical Engineering Co.,Ltd (herinafter referred to as Encode) was established in 1994, as one of the earliest
and largest manufacturers of in-vitro diagnostic field in China. Encode specializes in R&D, production and distribution of in-vitro diagnostic products. As a hi-tech and innovative enterprise, we have researched and developed series of microbiological diagnostic productds and immunology diagnostic rapid tests, which are used for diagnosis of infection disease, cardiac markers, tumor markers, fertility test, durgs of abuse and sexual transmitted disease.
Encode occupies a land of 15,000 m2 in Zhuhai. There are 2,000 GMP standard clean workshops of microbiology diagnostic products, colloidal gold diagnostic products and molecular with automatic manufacturing lines and passed the quality management system evaluation by SFDA and GDFDA.
Encode has established extensive cooperation with research centers in universtities, such as Lanzhou University, South China University of Technology, Jinan University and Shanghai Plumonary Hospital, etc. Whith strong R&D and technological innovation capability. Encode ranks leading level in terms of IVD industries field in China. We have also obtained ISO 13485:2003, ISO 9001:2001, CE and have been granted the title of national hi-tech enterprise.
Today, we provide medical products and health care solution to clients not only in China, but also worldwide, such as Europe, Africa, Middle East, South East, Asia etc.
With the experience and dedication of the scientific staffs, Zhuhai Encode Medical Engineering Co.,Ltd will continue our endeavor to provide professional in-vitro diagnostic products and health care solutions to the worldwide and make our contributions to the career of human health.
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