The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle and nuclear transport . Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. RCC1 (regulator of chromatin condensation 1) is the only known RanGEF . RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally . Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation and methylation . RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis . In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclinB/cdc2 . This phosphorylation may play a role in RCC1 interaction with chromatin and its RanGEF activity.

The antibody detects endogenous RCC1 protein.

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB: 1:500-10000, ELISA: 1:10000.

RCC1 Polyclonal Antibody product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.