PRINCIPLE
The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.

DETECTION PROCEDURE:
Mix Detection Reagents A & B at a 1:1 ratio.
Immediately add the mixture to the blot. Use 0.125 mL working solution per cm2 of membrane.
Incubate the blot for about 1 minute at room temperature.
Drain excess reagents.
Cover blot with a clear plastic sheet protector or clear plastic wrap.
Expose blot to X-ray film.

---

ECALBIO CO.,LTD    
Email: info@ecalbio.com     
Tel: 86-27-87745856    
Add : HZAU, Luoshi Road, Wuhan City 430000, China.

Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.

Ecalbio builds up professional platform to develop more than 10,000 products, including antibodies, antigens, biochemicals, ELISA kits, CLIA kits, and laboratory equipments,etc.

Ecalbio laboratories manufacture Antibodies, Proteins, Food safety ELISA kits, Food safety Rapid Tests, Animal Disease ELISA Kits, Animal Disease Rapid Test Kits, Laboratory equipments with incubators and diagnostic system.

Ecalbio has an enthusiastic and creative R&D team, consisting with a group of experienced technicians in lateral flow immunoassay field, and set up long relationship with first-class institutes,universities,academies, to provide excellent service for human healthy and earth.

Ecalbio are committed to saving lives by ensuring individuals worldwide have access to timely and correct diagnosis. Our products are used in over 100 countries worldwide by:
　Government Laboratories
　Food industry Laboratories
　Institutes
　Veterinary Clinics
　Pharmaceutical Industry.

Ecalbio welcomes potential partners and distributors to explorebusiness relationships with all customer in the worldwide, focused on scie-tech, integrity, quality and service for the customers.

ECALBIO CO.,LTD

HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
Website: www.ecalbio.com