AQR/IBP160 protein, also known as Aquarius homolog or Intron-binding protein of 160 kDa, is a spliceosomal intron-binding protein involved in pre-mRNA splicing and small nucleolar ribonucleoprotein (snoRNP) biogenesis. It links the position-dependent assembly of intron-encoded box C/D snoRNPs to pre-mRNA splicing, playing a key role in the folding of snoRNA sequences. This protein has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang. It binds to introns of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns during the late stages of splicing.