GFP is a protein of significant importance in biological research. Initially isolated from the jellyfish Aequorea victoria, GFP is composed of about 238 amino acids and has a molecular weight of approximately 27 kDa. It has the ability to absorb near-ultraviolet light (397nm) and emit green visible light (509nm), with its fluorescent structure formed spontaneously by cyclization and oxidation of the Ser65-Tyr66-Gly67 amino acid residues. It can be fused with other target genes to form chimeric proteins, allowing for quantitative analysis of the expression levels of the target genes, and visualizing their expression locations and quantity changes within tumor cells. This aids in exploring the role of genes in the occurrence and development of cancer and their molecular mechanisms.