dsRNA and ssRNA are two different types of RNA (ribonucleic acid), differing in their structure, function, and the types of viruses they are found in. dsRNA, or double-stranded RNA, refers to an RNA molecule with two complementary strands. This type of RNA is found in double-stranded RNA viruses. Additionally, dsRNA can be generated when exogenous genes such as viral genes, artificially transferred genes, or transposons randomly integrate into the host cell genome and are transcribed using the host cell machinery. These dsRNA molecules rapidly trigger a host cell response, which involves cleavage into multiple small RNA fragments of specific lengths and structures by the enzyme Dicer, known as siRNA. This RNA interference mechanism is highly conserved evolutionarily and effectively induces efficient and specific degradation of homologous mRNAs. Moreover, dsRNA technology serves as a biological tool for gene silencing and functional studies, enabling researchers to suppress the expression of target genes through RNAi mechanisms, thereby facilitating the investigation of gene function, expression patterns, and involvement in biological processes. ssRNA, or single-stranded RNA, refers to an RNA molecule consisting of only one strand. Most RNAs in organisms are single-stranded. While forming secondary structures, ssRNA can exhibit both single-stranded and double-stranded structural domains. Only certain RNA viruses consist of two complementary strands of RNA. ssRNA plays a crucial role in protein synthesis, serving as the template for transcription, a process where genetic information from DNA is transcribed into corresponding mRNAs. These mRNAs then undergo translation by ribosomes, converting the genetic information into amino acid sequences of proteins. Additionally, ssRNA is involved in regulating gene expression, such as through interactions with mRNAs by small RNAs, which significantly impact the adjustment of cellular and organismal functions.