14-3-3 beta is an important subtype within the 14-3-3 protein family, which consists of highly conserved, soluble acidic proteins exhibiting diverse biological functions. These proteins regulate numerous crucial cellular processes, including metabolism, cell cycle, cell growth and development, cell survival and apoptosis, as well as gene transcription. When the functions of these proteins become abnormal, they are closely associated with the occurrence of various diseases. Under the action of Ca(2+)/calmodulin-dependent protein kinase II, 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and strongly activate protein kinase c, potentially serving as multifunctional regulators of cell signaling processes modulated by kinases. Additionally, 14-3-3 proteins are localized in neurons and are transported to axonal nerve terminals, and they may also be present at lower levels in other eukaryotic tissues. In particular, 14-3-3 beta protein plays a pivotal role in multiple biological processes. Notably, due to its crucial role in cell signaling and regulation, 14-3-3 beta has become an important target for numerous disease studies.